Tuesday, july 28, 2015 a128 assessing performance for. Capillarys hba1c, but the vitros method generated results 4. The capillarys 2 flexpiercing instrument uses the principle of capillary electrophoresis in free solution. If you are looking for a book sebia capillarys 2 manual in pdf format, in that case you come on to right site. Hemoglobin fractions were determined by capillary electrophoresis sebia capillarys 2 flex piercing and high performance liquid chromatography hplc biorad variant ii. The capillarys hba 1c kit used on the capillarys 2 flex piercing system is designed for separating and quantifying the hba 1c fraction of hemoglobin while detecting hemoglobin variants and hba 2. The capillarys 2 flex piercing instrument has silica capillaries functioning in parallel allowing 8 simultaneous analyses for hba 1c quantification from whole blood sample. The minicap flex piercing offers all the features of the minicap, with the added ability to test whole blood. A the boxplots show the frequency of hba 2 in normal control. Capillarys hb a1c using the capillarys 2 flexpiercing. Measurement of hba and hba by capillarys 2 flex piercing hba. Octa has a large initial loading with 120 samples simultaneously.
Hemoglobin fractions were determined by capillary electrophoresis. Find new or used sebia capillarys2 flex piercing for sale on bimedis. Objectives we evaluated the biorad irvine, ca d100 and the sebia lisses, france capillarys 3 tera for the measurement of hemoglobin a1c hba1c in venous blood samples. Hba2 prime is a mild variant, but can be misleading. J065sebia capillarys 2 flex piercing maintenance expired from. Methods wholeblood samples and control material were analyzed with the d100 and capillarys 3 tera and compared with our routine method, hlc723g7 tosoh, tokyo, japan. The reagents capillarys immunotyping were previously cleared in submissions k042939 and k082085. This study presents the equivalence of both instruments capillarys 2 and capillarys 2 flex piercing. As with all flex piercing instruments, the capped tubes are gently inverted to ensure adequate mixing of whole blood samples. Pdf evaluation of the sebia capillarys 2 flex piercing. Conversely to hplc method, the capillary s electrophoresis technology allowed hbe and hb lepore fraction separation from hba2. Jaisson s, leroy n, meurice j, guillard e, gillery p. Sebia capillarys 2 flex piercing and high performance liquid chromatography hplc. Resultsthere was a good concordance between the results of capillary electrophoresis and hplc methods r2 0.
The aim of this study was to assess the performance of this new capillary electrophoresis kit for routine hba 1c quantification for patients with diabetes. The results showed that, in normal typing, carrier, thalassemia and hemoglobinopathies the percentage of hemoglobin a 2, f and e detected by capillarys 2 flex piercing and the two reference methods were not significantly different p0. Pdf evaluation of the sebia capillarys 2 flex piercing for. The applications of capillary electrophoresis in medical diagnostics have expanded considerably since the early 2000s. Measurement of hba1c and hba2 by capillarys 2 flex piercing. The measurement of imprecision in hba 2 and hbf quantification was verified on hba 2 cfp control and on three samples. Capillarys 2 flex piercing is an instrument utilizing capillary electrophoresis developed to be versatile while providing a clearcut and precise protein. F and e detected by capillarys 2 flex piercing and the two reference methods were not significantly different p 0.
Original papers a comparative evaluation of the analytical performances of capillarys 2 flex piercing, tosoh hlc723 g8, premier hb9210, and roche cobas c501 tinaquant gen 2 analyzers for hba1c determination xiaobin wu, yan chao, zemin wan, yunxiu wang, yan ma, peifeng ke, xinzhong wu, jianhua xu, junhua zhuang, xianzhang huang department of laboratory science, second affiliated. Este instrumento completamente automatico satisface. Evaluation of the sebia capillarys 2 flex piercing for the measurement of hba1c on venous and capillary blood samples. Navodila za uporabo instrumenta naj bodo zmeraj v blizini instrumenta. Performances of capillary electrophoresis and hplc methods in. Volume 20, issue 449, part 1, february 20, pages 4756. Four systems were used to obtain hba 1c measurements. Sebia introduced capillary electrophoresis as the fourth analytical principle capillarys 2 flex piercing c2fp. There was a good concordance between the results of capillary electrophoresis and hplc methods r2 0. The capillarys 2 flex piercing system provides the fastest hemoglobinopathy and thalassemia assay with up to 38 assays per hour. Sebia hba1c assay now has ce mark for diagnosis of. Cap piercing for improved workflow and biohazard precautions. This instrumentation replaces the capillarys 2 flex piercing. Wu x, chao y, wan z, wang y, ma y, ke p, wu x, xu j, zhuang j, huang x.
A comparative evaluation of the analytical performances of. Whole blood analysis for hemoglobin electrophoresis with no preanalytical preparation. We performed an evaluation of this system by checking imprecision, relative bias and robustness respect to the analysis of samples with variable total hemoglobin concentrations. The present study was conducted to evaluate the analytical performance and the organizational aspects of capillarys 2 flex piercing system cfp respect to agarose electrophoresis and hplc methods in hemoglobinopathies screening. Analytical evaluation of the capillarys 2 flex piercing. The goal of this study was to evaluate the integration of the new generation of automated capillary electrophoresis instrument, the capillarys 2 flex piercing sebia, in the daily practice of a specialized pathology laboratory. Needle designed to sample from capped tubes cap piercing. The capillarys hba1c kit implemented on the capillarys 2 flex piercing platform uses capillary electrophoresis to separate and quantify hba1c in human blood. All samples were analyzed by capillarys 2 flex piercing fp. Capillarys 2 flex piercing, flexibility for all laboratories. Analytical evaluation of the capillarys 2 flex piercing for.
The capillarys 2 flex piercing instrument is an automated analyzer which performs a complete hemoglobin profile for the quantitative analysis of the normal hemoglobin fractions a, a2 and f and for the detection of major hemoglobin variants s, c, e and d. Capillarys 2 and capillarys 2 flex piercing instruments and on sebia minicap instrument. Request pdf on dec 14, 2012, cas weykamp and others published hba1c. A comparative evaluation of the analytical performances of capillarys 2 flex piercing, tosoh hlc723 g8, premier hb9210, and roche cobas c501 tinaquant gen 2 analyzers for hba 1c determination. Capillarys 2 flex piercing se1227 kratka navodila za rokovanje z instrumentom. The correlation between capillarys 2 flex piercing and bio.
The horizontal line in each box represents the median values. Identification and quantification of hemoglobins in whole. The correlation between capillarys 2 flex piercing and biorad variant ii hplc system showed a linear correlation for hba2, hbf and hbs measurements, and the analysis interpretation was the same whatever the method used. The workflow study showed preparation time for the vitros averaged 4. The most sold capillary electrophoresis instrument worldwide. Octa runs primary capped tubes for whole blood with cap piercing and tube mixing.
Performance of the sebia capillarys 2 flex piercing. Capillarys 2 instrument compared to the capillarys 2 flex piercing instrumen, with serum and urine samples. We presented full variation of this book in epub, doc, txt, pdf, djvu forms. This multiparameter instrument offers a comprehensive menu on serum, urine and whole blood for protein, immunotyping, hba1c, hemoglobin and cdt carbohydrate deficient transferrin testing. Electro phoresis sebia minicap flex piercing wholesale. Sebia, lisses, france for measurement of hemoglobin a1c. To provide flexibility and instrument consolidation, the capillarys 2 flex piercing also offers, in addition to hemoglobin and hba1c testing, a complete test menu to include serum and urine proteine6, immunotyping. The minicap flex piercing instrument offers flexibility for small to medium size laboratories. Pdf evaluation of the sebia capillarys 3 tera and the bio.
The aim of this study was to assess the performance of this new capillary electrophoresis kit for routine hba 1c quantification for patients with. Measurement of hba and hba by capillarys 2 flex piercing. Capillarys immunotyping pn2100 and itif control pn 4788 using the capillarys 2 instrument pn 1222 and capillarys 2 flex piercing instrument pn 1227 g. Capillarys hb a1c using the capillarys 2 flexpiercing instrument. First evaluation of capillarys 2 flex piercing sebia as a. The capillarys 2 flex piercing instrument uses the principle of capillary electrophoresis in free solution. Pdf evaluation of the sebia capillarys 2 flex piercing for the. Oct 15, 2017 the values of haemoglobin a 2 hba 2 were analyzed by capillarys 2 flex piercing for normal controls and thalassaemia. Hba1c is a key biomarker for the monitoring of glycemic balance in diabetic patients. This study was conducted to evaluate the ce methodology using sebias capillarys 2 flex piercing as a complementary method to hplc. Sebia now offers a clearcut and precise hba1c test on capillarys 3 tera, capillarys 2 flex piercing and minicap flex piercing. Sebia focus sebia sebia hba1c assay set up on two capillarys 2 flex piercing in the arnaudbiolys a practical guide for the interpretation and sebia focus inserm n 2.
Pdf a comparative evaluation of the analytical performances. Capillarys 2 flex piercing the reference in routine capillary electrophoresis for medical laboratories. It may be measured by various methods, including hplc and immunoassays. Identification of haemoglobin new york by haemoglobin a. The values of haemoglobin a 2 hba 2 were analyzed by capillarys 2 flex piercing for normal controls and thalassaemia. In 2015, the company launched the highspeed, high resolution automation electrophoresis program, capillarys 3. Dec 24, 2019 capillarys 2 flex piercing sebia capillarys 2 neonat fast sebia capillarys 3 tera sebia capillarys 3 octa sebia minicap flex piercing sebia gel electrophoresis assist sebia gelscan sebia hydrasys 2 sebia hydrasys 2 scan sebia hydrasys 2 focusing sebia hydrasys 2 scan focusing sebia elisa test kits elisa hepatitis dia. Evaluation of the sebia capillarys 2 flex piercing for the. Original papers a comparative evaluation of the analytical performances of capillarys 2 flex piercing, tosoh hlc723 g8, premier hb9210, and roche cobas c501 tinaquant gen 2 analyzers for hba1c determination xiaobin wu, yan chao, zemin wan, yunxiu wang, yan ma, peifeng ke, xinzhong wu, jianhua xu, junhua zhuang, xianzhang huang department of laboratory science, second affiliated hospital of. Principe du test lelectrophorese des proteines du serum humain est une analyse tres utile en laboratoire danalyses cliniques pour rechercher les modifications du. We report a case of an extremely rare hemoglobin hb varianthb broomhill, which has been only reported once in the literature.
The capillarys 2 flex piercing instrument has capillaries functioning in parallel allowing 8 simultaneous analyses for hemoglobin quantification from whole blood sample. First evaluation of capillarys 2 flex piercing sebia as a new analyzer for hba assay by capillary electrophoresis antioxidant and oligonutrient status, distribution of amino acids, muscle damage, inflammation, and evaluation of renal function in elite rugby players. Heylen o1, van neyghem s1, exterbille s1, wehlou c1, gorus f1, weets i 2. Rad variant ii hplc system showed a linear correlation for hba2, hbf and hbs measurements, and the analysis interpretation was the same whatever the method used. Capillarys 2 flex piercing detected a rare case of hb. Sebia capillarys 2 flex piercing full automation with cap piercing technology. Perkinelmer to serve as exclusive global perkinelmer to serve as exclusive global distributor for sebias hemoglobin newborn screening system from manual to automated. Capillarys 2 flex piercing is an instrument utilizing capillary electrophoresis developed to be versatile while providing a clearcut and precise protein separation with high throughput. Capillarys is fitted with 8 capillaries, making it possible to carry out 8. Capillarys immunotyping urine capillarys hba1c capillarys urine venouscapillary blood technology advancements. Pdf backgroundaim glycated hemoglobin hba1c is considered the gold standard for assessing the compensation and treatment of diabetes. Sebia hydragel if, hydragel if penta, hydragel bence jones standard mask and dynamic mask performed using the hydrasys and hydrasys 2 instruments and the k20 electrophoresis chamber.
Pdf evaluation of sebia capillarys 2 flex piercing for the. Capillarys 2 flex piercing detected a rare case of hb broomhill. Sebia, lisses, france, and its first evaluation was. The fully automated instrument meets the needs of the laboratory while providing clearcut and precise separations utilizing the capillary electrophoresis technology. Pred uporabo dobro preberi tudi originalna navodila, posebej za uporabo vseh moznih funkcij.
Zone electrophoresis ce method sebias capillarys 2 flex piercing has emerged as a very robust separation technique that provides better separation for variants eluting with hba2. This multiparameter instrument offers a comprehensive menu on serum, urine and whole blood for protein, immunotyping, hba1c, hemoglobin and cdt carbohydrate. Start date 01 feb, 2021 1 month ago due date 03 feb, 2021 1 month ago opportunity type prebid notification. Serum, urine protocol currently under validation, whole blood capped tubes true innovation. A comparative evaluation of the analytical performances of capillarys 2 flex piercing, tosoh hlc723 g8, premier hb9210, and roche cobas c501 tinaquant gen 2 analyzers for hba 1c determination xiaobin wu, yan chao, zemin wan, yunxiu wang, yan ma, peifeng ke, xinzhong wu, jianhua xu, junhua zhuang, xianzhang huang. Sep 07, 2020 capillarys 3 octa 8 capillaries minicap flex piercing 2 capillaries each sample is diluted in a dilution buffer and the capillaries are filled with the separation buffer. Highresolution hba1c separation and hemoglobinopathy. Serum, urine, whole blood capped tubes o comprehensive test menu. An evaluation protocol to test precision, trueness. A sample dilution with hemolysing solution is prepared and injected by aspiration at the anodic end of the capillary. We evaluated the sebia capillarys 2 flex piercing cap 2fp. Recent studies have been published regarding the performance of the recently commercially released sebia capillarys 2 flex piercing analyzer sebia cap 2fp.
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