Leica bond rx is part of a complete research solution that. Ensure the correct update is applied commensurate with your software version and region. Nanostring will introduce the geomx dsp to the leica biosystems bond rx user community on may 16 th, 2019 at a leica biosystems conference in san diego, ca. Niroshan ramachandran will highlight the workflow in a talk entitled, morphology driven highplex spatial analysis of ffpe tissue microenvironments.
About this guide this user manual provides guidelines and protocols to use the rnascope 2. Then select the correct label refer to the bond rx user manual for further information. The approach involves detection with opal reactive fluorophores, followed by heatinduced stripping steps for. Opal polaris 7color automation ihc kit 50 slides nel871001kt. Ubiquitin c and dapb were used as positive and negative controls, respectively. Bond rx product includes bond rxm and all associated software and. This user manual provides guidelines and protocols to use the basescopels reagent kit with the leica biosystems bond rx research advanced staining system. Leica geosystems rx1200 user manual pdf download manualslib. Histocore microtomes leica biosystems pdf catalogs. According to your specific instrument type, select either the bond rx or bond rxm tab. Accelerate test programs through speed, efficiency, and consistent automation, reducing manual work so that researchers can spend less time at the bench and more time innovating. Leica biosystemsmaking multiplex easy multiplex is the identification of several targets on the same slide.
All protocols and protocol templates developed in conjunction with our oi partners are available on bond rx and bond rx m so you can access the latest research advances in the shortest time. You may also rightclick on the scanned label images, and select. Researchfocused software the bond rxm user interface has a workflow and terminology tailored for research. Leica bond rx is available immediately in north america through authorized leica microsystems representatives. Open the catalog to page 3 eica biosystems researchfocused software the bond rxm user interface has a workflow and terminology tailored for research. Available name descriptionformat of rx1200 manuals documentation user manual all instructions required in order to operate the product to a basic level are contained in the user manual. Product description the viewrna ezl detection kit1plex red contains the universal reagents that are required to. Bond rx materials required the opal 4color and 7color automation ihc assay require the following materials and equipment that are available only from leica biosystems and are listed in table 2. Leica pdf user manuals, service manuals, operating. Increase your test menu with novocastra antibodies and probes backed by leicas extensive inhouse expertise. Perkinelmer and leica biosystems have entered into a nonexclusive co marketing.
Automated ihc inmmunohistochemistry and ish in situ hybridization system. Biosystems technical data type of microtome general histocore biocut mechanical fully motorized dimensions and weight width including handwheel and coarse feed wheel depth including the section waste tray height without top tray w x d x h. Do not apply this update to bond rx or bond rxm research systems. Bond research detection systems and open containers will need to be registered periodically, but not for every run. Leica microsystems is a world leader in microscopes and scientific instruments. These are most commonly proteins ihc, if, but may also be rna and dna. In developing the bond iii, we focused on end user interaction to incorporate a range of highefficiency features for less user intervention. We recommend that all bond systems be updated to the latest software version. Designed with the user in mind, everything from immediate reagent access. Ffpe tissue sections were baked and deparaffinized on the instrument, followed by epitope retrieval using leica epitope retrieval buffer 2 at 95c or at 88c for 15 min and protease treatment 15 min at 40c. Set up the bond rx to set up the bond rx autostainer, you must register any reagents to be used, as well as the detection system and containers they will be loaded in. Ms5, mz6, mz75, mz95, mz125, mz16, mz16 a, mz16 fa. Operating leica bond rx refer to the leica bond rx user manual for information about chemical and mechanical hazards, electrical hazards, and additional cautionary information.
Leica bond rx is effectively three stainers in one instrument. Register 10% nbf, nbf stop buffer, and enzyme 1 to titration containers. The complete solution to high quality tissue processing traceability track and trace integrated bar code scanner captures basket id, user id and lot number eliminating manual records, while providing a higher level of specimen safety with traceability. Leica biosystems bond rx system instructions for use. All bond rx specific reagents, such as bulk buffers and the research detection kit user fillable vials are provided from leica. So you have the flexibility of continuous processing while keeping your slides together to reduce sorting. The researchfocused software is easy to learn, new assays are quick to set up and the incredibly flexible software makes for straightforward assay optimization and customization. Traci degeer global product and innovation managerbond rx leica biosystemsspeaker biography. Basescope ls detection reagent kit user manual for bdz 11 or. Operating leica biosystems bond iii advanced staining system refer to the leica bond user manual for information about chemical an d mechanical hazards, electrical hazards, and additional cautionary information.
Leica m series user manual 3 function of the microscopes the leica m125 b and leica m165 c b microscopes, for which this user manual has been written, are designed for routine examinations of cell and tissue cultures, liquids, and sediments. A user manual is available from perkinelmer to assist you in setting up your. For detailed contact information on your nearest sales office or distributor please visit our website. The basescopels assays are compatible with a variety of sample types. Primary antibody binding to tissue sections can be visualized using bond polymer refine detection, where it provides intense, high resolution staining. Once all slides and reagents have been reconciled, click the start button under the slide tray image on the screen to begin the run.
Robust detection of rna biomarkers for drug safety assessment. This includes examining specimens taken from the human body for the purpose. The tray is registered on bond like any other detection system one barcode only, but each of the containers can be configured with reagent of the user s choice. Page 1 leica rx1200 user manual draft copy only version 5. Refer to your leica biosystems bond rx user manual, accessible from the help icon in the instrument software, for additional information. As a result, you have many flexible solutions for your research. Technical data histocore biocut r histocore multicut r histocore autocut r histocore nanocut r type of microtome mechanical semi motorized fully motorized fully motorized width including handwheel and coarse feed wheel depth including the section waste tray height without top tray w xd xh. Application of probe, counterstain and cover slipping are the only manual st. Bond research detection system 2 ds9777, this open detection system consists of nine standard 30ml open containers in a reagent tray.
Differences between the various models manual are marked and described. Bond ihc polymer detection kit ds9800 leica biosystems. Bond is a trademark of leica biosystems melbourne pty. Leica autostainer xl instructions for use manual pdf download. Multiplex fluorescence immunohistochemistry using the ultivue.
Five micrometers sections were stained for immunofluorescence on a leica bond rx leica biosystems, buffalo grove, il with antivimentin mouse monoclonal 0. Leica biosystems partners with a range of open innovation oi partners. The slides were independently evaluated by three separate observers fzm, ar and rf. Storage bond titration kit opt9049 room temperature 2025 c. Leica biosystems an international company with a strong network of worldwide customer services. Leica biosystems continues to advance cancer diagnostics with the new bond max. After scanning the slides, select the play triangular button on the screen located under the start tray.
Leica bondmax automatic staining immunohistochemistry. Fully automated ihc and ish leica bondiii from leica. Sep 10, 2014 operating leica bond rx refer to the leica bond rx user manual for information about chemical and mechanical hazards, electrical hazards, and additional cautionary information. We recommend reading the entire user manual before beginning any protocols. The sections were pretreated with a leica bond er2 buffer for 20. Bond research detection detection systems leica biosystems. What reagents are provided by akoya biosciences and which are provided by leica biosystems. Nanostring and leica biosystems to comarket geomx digital. The country specific power cord needs to be ordered separately. Leica bond iii, the fully automated ihc and ish stainer that delivers what really matters. Required user maintenance daily, weekly daily, weekly. With the emphasis on deriving maximum information from limited tissue, multiplex allows the researcher.
Place the tray in the leica bond rx, and press the button to load the tray onto the machine. For highsensitivity staining, select bond refine dab or red and chromoplex 1 double stain detection systems. Researchfocused software the bond rx user interface has a workflow and terminology tailored for research. The opal signal is largely unaffected by stripping and antibody removal. Continuous as your research program grows, so does leica bond. The bond rx fully automated research stainer from leica biosystems provides superior quality and flexibility whilst enabling the automation of ihc, ish and emerging tests. Leica bond iii is up to 50% faster than previous generation stainers requires virtually no daily maintenance classleading quality with superior bond reagents and proven covertile technology. Mar 14, 20 leica m series user manual 3 function of the microscopes the leica m125 b and leica m165 c b microscopes, for which this user manual has been written, are designed for routine examinations of cell and tissue cultures, liquids, and sediments. Enjoy more time for academic research with bond rxm fully. With the ability to connect up to five leica bond rx instruments to a single data collection.
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